Interaction Details


Details of experiments between Q6ZTQ3 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.65
Normalized holdup BI: 0.84
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.48
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.48
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.04
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.58
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 29.54
Standard deviation of dissociation constant (10-6M): 3.38
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.53
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.32
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.39
PUBMED ID: 36115835