Interaction Details


Details of experiments between Q6ZMN7 and Q8N103
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TAGAP
Fragment: 722-731
Site: PBM
Sequence: QFQYAKESYI
Construct: biotin-ttds-QFQYAKESYI
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.14
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.96
PUBMED ID: 36115835