Interaction Details


Details of experiments between Q6ZMN7 and P27816
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAP4
Fragment: 1143-1152
Site: PBM
Sequence: LDSQIQETSI
Construct: biotin-ttds-LDSQIQETSI
Average holdup BI: 0.52
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.52
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.84
PUBMED ID: 36115835