Interaction Details

Details of experiments between Q6ZMN7 and P11166
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC2A1
Fragment: 483-492
Site: PBM
Sequence: FHPLGADSQV
Construct: biotin-ttds-FHPLGADSQV

Average holdup BI: 0.02

Holdup BI standard deviation: 0.05

Normalized holdup BI: 0.02

Normalized holdup BI standard deviation: 0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 4

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835