Interaction Details


Details of experiments between Q6ZMN7 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: 0.7
Normalized holdup BI: 0.78
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.38
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.73
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.73
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.25
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 5.94
Standard deviation of dissociation constant (10-6M): 0.24
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.23
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 6
Standard deviation of dissociation constant (10-6M): 1.5
Experiment method: SPR
Number of measurements: 1
Measured pKd: 5.22
PUBMED ID: 30726710

Experiment series 5

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.62
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
Measured pKd: 5.12
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.53
Normalized holdup BI: 0.63
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.05
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 7

Protein A

Protein: PDZRN4
Fragment: 389-498
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.01
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 6
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: PDZRN4
Fragment: 389-498
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: 0.24
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.26
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.09
Normalized holdup BI: -0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Modification: (TPO)156
Sequence: SSRTRRETQL
Construct: Biotin-ttds-SSRTRREpTQL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835