Interaction Details


Details of experiments between Q6ZMN7 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.29
Normalized holdup BI: 0.29
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.3
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Measured dissociation constant (10-6M): 105
Standard deviation of dissociation constant (10-6M): 8.49
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 3.98
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PDZRN4
Fragment: 389-498
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: -0.06
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835