Interaction Details


Details of experiments between Q6ZMN7 and O14734
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACOT8
Fragment: 310-319
Site: PBM
Sequence: VKPQVSESKL
Construct: biotin-ado-ado-VKPQVSESKL
Average holdup BI: 0.1
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.