Interaction Details


Details of experiments between Q6UXZ0 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.82
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.82
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 3
Measured pKd: 5.49
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 4.37
Standard deviation of dissociation constant (10-6M): 0.1
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.36
PUBMED ID: 36115835