Interaction Details


Details of experiments between Q6UXZ0 and Q14160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SCRIB
Fragment: 999-1093
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.56
Normalized holdup BI: 0.62
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.01
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Holdup BI standard deviation: 0.1
Normalized holdup BI: 0.42
Normalized holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 4.64
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SCRIB
Fragment: 718-814
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 24.42
Standard deviation of dissociation constant (10-6M): 1.67
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SCRIB
Fragment: 858-949
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.22
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.23
PUBMED ID: 36115835