Interaction Details


Details of experiments between Q6UXZ0 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.07
PUBMED ID: 36115835