Interaction Details


Details of experiments between Q6UXZ0 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMIGD1
Fragment: 253-262
Site: PBM
Sequence: DKDPHSETAL
Construct: biotin-ttds-DKDPHSETAL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.03
PUBMED ID: 36115835