Interaction Details


Details of experiments between Q6UVM3 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 0.22
Standard deviation of dissociation constant (10-6M): 0.02
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 6.66
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: KCNT2
Fragment: 1126-1135
Site: PBM
Sequence: GQDSREETQL
Construct: biotin-ttds-GQDSREETQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.89
Normalized holdup BI: 0.89
Immobilized partner concentration (10-6M): 13.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.91
PUBMED ID: 36115835