Interaction Details


Details of experiments between Q6PCD5 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RFWD3
Fragment: 1-774
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein
Average holdup BI: 0.53
Immobilized partner concentration (10-6M): 26
P value: 3.11
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
Measured pKd: 4.64
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18