Interaction Details


Details of experiments between Q6P1L5 and P27348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FAM117B
Fragment: 204-213
Site: 14-3-3_pSer209
Modification: (SEP)209
Sequence: TRQPSSSPSS
Construct: TRQPS(pSer)SPSS (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: -0.33
Holdup BI standard deviation: 1.06
Immobilized partner concentration (10-6M): 130
P value: 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: FAM117B
Fragment: 215-224
Site: 14-3-3_pSer220
Modification: (SEP)220
Sequence: IRRTSSLDTL
Construct: IRRTS(pSer)LDTL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.69
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 8.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.24
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: FAM117B
Fragment: 386-395
Site: 14-3-3_pSer391
Modification: (SEP)391
Sequence: SSSTRSIDTQ
Construct: SSSTR(pSer)IDTQ (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: -0.14
Holdup BI standard deviation: 0.31
Immobilized partner concentration (10-6M): 130
P value: 0.51
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: FAM117B
Fragment: 33-42
Site: 14-3-3_pThr38
Modification: (TPO)38
Sequence: QPMRATVPFQ
Construct: QPMRA(pThr)VPFQ (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.79
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 5.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.45
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: FAM117B
Fragment: 451-460
Site: 14-3-3_pThr456
Modification: (TPO)456
Sequence: LPKYATSPKP
Construct: LPKYA(pThr)SPKP (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.91
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 130
P value: 6.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.87
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))