Interaction Details


Details of experiments between Q6P0Q8 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF3
Fragment: 510-519
Site: PBM
Sequence: SSSNSEDTEM
Construct: Biotin-Ahx-SSSNSEDTEM
Average holdup BI: 0.35
Holdup BI standard deviation: 0
Normalized holdup BI: 0.35
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.5
PUBMED ID: 36115835