Interaction Details


Details of experiments between Q6P0Q8 and Q15599
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF2
Fragment: 328-337
Site: PBM
Sequence: NRKREIFSNF
Construct: Biotin-Ahx-NRKREIFSNF
Average holdup BI: 0.12
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.12
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.89
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.