Interaction Details


Details of experiments between Q6P0Q8 and Q15311
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.7
Normalized holdup BI: 0.7
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.19
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.66
Normalized holdup BI: 0.66
Immobilized partner concentration (10-6M): 28
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.88
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Measured dissociation constant (10-6M): 27.8
Standard deviation of dissociation constant (10-6M): 1.05
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.56
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RALBP1
Fragment: 646-655
Site: PBM
Sequence: PSRDRKETSI
Construct: biotin-ttds-PSRDRKETSI
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835