Interaction Details


Details of experiments between Q6P0Q8 and P27037
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Average holdup BI: 0.77
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.05
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ACVR2A
Fragment: 504-513
Site: PBM
Sequence: VDFPPKESSL
Construct: biotin-ttds-VDFPPKESSL
Measured dissociation constant (10-6M): 11.51
Standard deviation of dissociation constant (10-6M): 0.3
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.94
PUBMED ID: 36115835