Interaction Details


Details of experiments between Q6P0Q8 and P26554
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.77
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.27
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Measured dissociation constant (10-6M): 7.63
Standard deviation of dissociation constant (10-6M): 0.21
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.12
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV51-E6
Fragment: 142-151
Site: PBM
Sequence: RTRQRNETQV
Construct: biotin-ttds-RTRQRNETQV
Average holdup BI: 0.42
Immobilized partner concentration (10-6M): 7.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.07
PUBMED ID: 36115835