Interaction Details


Details of experiments between Q6P0Q8 and P06427
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.8
Holdup BI standard deviation: 0.08
Normalized holdup BI: 0.8
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.37
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.32
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Measured dissociation constant (10-6M): 6.16
Standard deviation of dissociation constant (10-6M): 0.48
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.21
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SRSRRRETAL
Construct: Biotin-ttds-SRSRRREpTAL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
PUBMED ID: 36115835