Interaction Details


Details of experiments between Q68EM7 and Q13884
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL

Protein B

Protein: SNTB1
Fragment: 107-196
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Normalized holdup BI: 0.19
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.13
PUBMED ID: 36115835