Interaction Details


Details of experiments between Q68EM7 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP17
Fragment: 662-676
Site: PxxP_2
Sequence: HPPSLSPKPPTRSPS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.15
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.99
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: ARHGAP17
Fragment: 662-676
Site: PxxP_2
Sequence: HPPSLSPKPPTRSPS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.15
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 3.99
PUBMED ID: N

Experiment series 3

Protein A

Protein: ARHGAP17
Fragment: 662-676
Site: PxxP_2
Sequence: HPPSLSPKPPTRSPS
Construct: Bio E E Ado Ado-HPPSLSPKPPTRSPS-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: ARHGAP17
Fragment: 1-881
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.23
Immobilized partner concentration (10-6M): 10
P value: 3.24
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 5

Protein A

Protein: ARHGAP17
Fragment: 632-646
Site: PxxP_1
Sequence: AVKKPAPAPPKPGNP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: ARHGAP17
Fragment: 632-646
Site: PxxP_1
Sequence: AVKKPAPAPPKPGNP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 7

Protein A

Protein: ARHGAP17
Fragment: 765-779
Site: PxxP_3
Sequence: PLGKQNPSLPAPQTL
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: ARHGAP17
Fragment: 765-779
Site: PxxP_3
Sequence: PLGKQNPSLPAPQTL
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 9

Protein A

Protein: ARHGAP17
Fragment: 801-815
Site: PxxP_4
Sequence: PKPRNRPSVPPPPQP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: ARHGAP17
Fragment: 801-815
Site: PxxP_4
Sequence: PKPRNRPSVPPPPQP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N