Interaction Details


Details of experiments between Q68EM7 and O15018
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP17
Fragment: 872-881
Site: PBM
Sequence: IDNDTESTAL
Construct: biotin-ttds-IDNDTESTAL

Protein B

Protein: PDZD2
Fragment: 326-424
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.11
PUBMED ID: 36115835