Interaction Details


Details of experiments between Q68DX3 and Q96DR7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: Biotin-ado-ado-GRLLGLETNV
Average holdup BI: 0.29
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.49
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: biotin-ttds-GRLLGLETNV
Average holdup BI: 0.2
Normalized holdup BI: 0.22
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.22
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: biotin-ttds-GRLLGLETNV
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: Biotin-ado-ado-GRLLGLETNV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: biotin-ttds-GRLLGLETNV
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF26
Fragment: 862-871
Site: PBM
Sequence: GRLLGLETNV
Construct: Biotin-ado-ado-GRLLGLETNV
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6