Interaction Details


Details of experiments between Q68DX3 and Q5KSL6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKK
Fragment: 1262-1271
Site: PBM
Sequence: DPLTPSRSQL
Construct: biotin-ttds-DPLTPSRSQL
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835