Interaction Details


Details of experiments between Q68DX3 and O14924
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-ado-ado-KTSAHHATFV
Average holdup BI: 0.44
Normalized holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD2
Fragment: 944-1038
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-Ahx-KTSAHHATFV
Average holdup BI: 0.42
Normalized holdup BI: 0.46
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-ado-ado-KTSAHHATFV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: FRMPD2
Fragment: 1073-1166
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-Ahx-KTSAHHATFV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-ado-ado-KTSAHHATFV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: FRMPD2
Fragment: 770-861
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RGS12
Fragment: 1438-1447
Site: PBM
Sequence: KTSAHHATFV
Construct: Biotin-Ahx-KTSAHHATFV
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835