Interaction Details


Details of experiments between Q68D42 and Q96JH8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMEM215
Fragment: 226-235
Site: PBM
Sequence: QGRWDHETIV
Construct: Biotin-Ahx-QGRWDHETIV

Protein B

Protein: RADIL
Fragment: 973-1075
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.52
Normalized holdup BI: 0.52
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.83
PUBMED ID: 36115835