Interaction Details


Details of experiments between Q5VV41 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF16
Fragment: 700-709
Site: PBM
Sequence: MERLRVETDV
Construct: biotin-ttds-MERLRVETDV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.34
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGEF16
Fragment: 700-709
Site: PBM
Sequence: MERLRVETDV
Construct: biotin-ttds-MERLRVETDV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 36115835