Interaction Details


Details of experiments between Q5VV41 and P31947
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGEF16
Fragment: 102-111
Site: 14-3-3_pSer107
Modification: (SEP)107
Sequence: PARHQSFGAA
Construct: PARHQ(pSer)FGAA (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.2
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 113
P value: 1.79
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: ARHGEF16
Fragment: 102-111
Site: 14-3-3_pSer107
Modification: (SEP)107
Sequence: PARHQSFGAA
Construct: PARHQ(pSer)FGAA (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.19
Holdup BI standard deviation: 0.48
Immobilized partner concentration (10-6M): 142
P value: 0.39
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: ARHGEF16
Fragment: 487-496
Site: 14-3-3_pSer492
Modification: (SEP)492
Sequence: FSKVKSLPLI
Construct: FSKVK(pSer)LPLI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.79
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 142
P value: 4.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.41
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: ARHGEF16
Fragment: 487-496
Site: 14-3-3_pSer492
Modification: (SEP)492
Sequence: FSKVKSLPLI
Construct: FSKVK(pSer)LPLI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.69
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 113
P value: 2.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.3
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: ARHGEF16
Fragment: 1-10
Site: 14-3-3_pSer6
Modification: (SEP)6
Sequence: MAQRHSDSSL
Construct: MAQRH(pSer)DSSL (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.33
Holdup BI standard deviation: 1.11
Immobilized partner concentration (10-6M): 142
P value: 0.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: ARHGEF16
Fragment: 102-111
Site: 14-3-3_pSer107
Modification: (SEP)107
Sequence: PARHQSFGAA
Construct: PARHQ(pSer)FGAA (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: -0.23
Holdup BI standard deviation: 0.76
Immobilized partner concentration (10-6M): 111
P value: 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 7

Protein A

Protein: ARHGEF16
Fragment: 487-496
Site: 14-3-3_pSer492
Modification: (SEP)492
Sequence: FSKVKSLPLI
Construct: FSKVK(pSer)LPLI (crude synthetic peptide)

Protein B

Protein: SFN
Fragment: 1-248
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3sigma
Average holdup BI: 0.76
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 111
P value: 4.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.46
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))