Interaction Details


Details of experiments between Q5THJ4 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: VPS13D
Fragment: 1719-1728
Site: 14-3-3_pSer1724
Modification: (SEP)1724
Sequence: PDMPRSLPSH
Construct: PDMPR(pSer)LPSH (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.44
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 116
P value: 3.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.83
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: VPS13D
Fragment: 1719-1728
Site: 14-3-3_pSer1724
Modification: (SEP)1724
Sequence: PDMPRSLPSH
Construct: PDMPR(pSer)LPSH (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.4
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 143
P value: 4.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: VPS13D
Fragment: 2450-2459
Site: 14-3-3_pSer2455
Modification: (SEP)2455
Sequence: VTKRSSLPVS
Construct: VTKRS(pSer)LPVS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.33
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 116
P value: 4.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.63
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: VPS13D
Fragment: 658-667
Site: 14-3-3_pSer663
Modification: (SEP)663
Sequence: GFGYQSELEL
Construct: GFGYQ(pSer)ELEL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.25
Holdup BI standard deviation: 0.37
Immobilized partner concentration (10-6M): 116
P value: 0.62
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))