Interaction Details


Details of experiments between Q5TCZ1 and P27348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SH3PXD2A
Fragment: 997-1006
Site: 14-3-3_pSer1002
Modification: (SEP)1002
Sequence: LRRNESLTAT
Construct: LRRNE(pSer)LTAT (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0
Holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 130
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: SH3PXD2A
Fragment: 1012-1021
Site: 14-3-3_pSer1017
Modification: (SEP)1017
Sequence: VRRNSSFSTA
Construct: VRRNS(pSer)FSTA (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.16
Holdup BI standard deviation: 0.19
Immobilized partner concentration (10-6M): 130
P value: 0.81
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SH3PXD2A
Fragment: 1047-1056
Site: 14-3-3_pSer1052
Modification: (SEP)1052
Sequence: PAQRNSIPVS
Construct: PAQRN(pSer)IPVS (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 130
P value: 1.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: SH3PXD2A
Fragment: 374-383
Site: 14-3-3_pSer379
Modification: (SEP)379
Sequence: AKKEISLPIL
Construct: AKKEI(pSer)LPIL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.65
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 4.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.15
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: SH3PXD2A
Fragment: 415-424
Site: 14-3-3_pSer420
Modification: (SEP)420
Sequence: QRAQISSPNL
Construct: QRAQI(pSer)SPNL (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.53
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 6.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.94
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: SH3PXD2A
Fragment: 251-260
Site: 14-3-3_pThr256
Modification: (TPO)256
Sequence: LDRRWTLGGM
Construct: LDRRW(pThr)LGGM (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.87
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 2.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.69
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: SH3PXD2A
Fragment: 513-522
Site: 14-3-3_pThr518
Modification: (TPO)518
Sequence: RTSTLTRPKV
Construct: RTSTL(pThr)RPKV (crude synthetic peptide)

Protein B

Protein: YWHAQ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3tau
Average holdup BI: 0.04
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 130
P value: 0.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))