Interaction Details


Details of experiments between Q5TCQ9 and Q7Z628
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV
Average holdup BI: 0.9
Normalized holdup BI: 0.95
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.13
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.83
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.92
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.92
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.96
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 5.91
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.81
Normalized holdup BI: 0.89
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.74
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 5

Protein A

Protein: MAGI3
Fragment: 847-941
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 3.72
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI3
Fragment: 1014-1103
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI3
Fragment: 572-655
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI3
Fragment: 716-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: -0
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835