Interaction Details


Details of experiments between Q5TCQ9 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.69
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.76
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: Biotin-ado-ado-RLPKIKETTV
Average holdup BI: 0.83
Normalized holdup BI: 0.88
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.68
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.7
Holdup BI standard deviation: 0
Normalized holdup BI: 0.77
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 21.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.27
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI3
Fragment: 1-114
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI3
Fragment: 1014-1103
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI3
Fragment: 572-655
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI3
Fragment: 716-815
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI3
Fragment: 847-941
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835