Interaction Details


Details of experiments between Q5TCQ9 and Q14500
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNJ12
Fragment: 424-433
Site: PBM
Sequence: QRPYRRESEI
Construct: biotin-ttds-QRPYRRESEI
Average holdup BI: 0.43
Normalized holdup BI: 0.47
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.75
PUBMED ID: 36115835