Interaction Details

Details of experiments between Q5TCQ9 and P11166
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MAGI3
Fragment: 398-503
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLC2A1
Fragment: 483-492
Site: PBM
Sequence: FHPLGADSQV
Construct: biotin-ttds-FHPLGADSQV

Average holdup BI: 0.04

Holdup BI standard deviation: 0.01

Normalized holdup BI: 0.05

Normalized holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835