Interaction Details


Details of experiments between Q5T5U3 and Q9UDY2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TJP2
Fragment: 1181-1190
Site: PBM
Sequence: QSARYRDTEL
Construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TJP2
Fragment: 1181-1190
Site: PBM
Sequence: QSARYRDTEL
Construct: biotin-ttds-QSARYRDTEL
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 36115835