Interaction Details


Details of experiments between Q5T5U3 and Q7Z6J2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835