Interaction Details


Details of experiments between Q5T5U3 and O75052
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NOS1AP
Fragment: 497-506
Site: PBM
Sequence: GDGLDDEIAV
Construct: biotin-ado-ado-GDGLDDEIAV
Average holdup BI: 0.35
Normalized holdup BI: 0.35
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.51
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.