Interaction Details


Details of experiments between Q5T2W1 and Q9Y2H0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.69
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.73
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.25
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.19
Normalized holdup BI: 0.19
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.14
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.87
PUBMED ID: 36115835