Interaction Details


Details of experiments between Q5T2W1 and P36762
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.49
Normalized holdup BI: 0.53
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.86
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.2
Normalized holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.18
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV49-L2
Fragment: 512-521
Site: PBM
Sequence: RRRKRKRTYL
Construct: Biotin-Ahx-RRRKRKRTYL
Average holdup BI: 0.15
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4
PUBMED ID: 36115835