Interaction Details


Details of experiments between Q5T2W1 and O43572
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AKAP10
Fragment: 653-662
Site: PBM
Sequence: DQPLEKSTKL
Construct: biotin-ado-ado-DQPLEKSTKL
Average holdup BI: 0.89
Normalized holdup BI: 0.94
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 6.02
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AKAP10
Fragment: 653-662
Site: PBM
Sequence: DQPLEKSTKL
Construct: biotin-ado-ado-DQPLEKSTKL
Average holdup BI: 0.88
Normalized holdup BI: 0.88
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.69
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: AKAP10
Fragment: 653-662
Site: PBM
Sequence: DQPLEKSTKL
Construct: biotin-ado-ado-DQPLEKSTKL
Average holdup BI: 0.5
Normalized holdup BI: 0.5
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.79
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.