Interaction Details


Details of experiments between Q5T2W1 and O43182
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.36
Normalized holdup BI: 0.38
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.55
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGAP6
Fragment: 965-974
Site: PBM
Sequence: NPDALPETLV
Construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835