Interaction Details

Details of experiments between Q5T2T1 and Q9NZH5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: MPP7
Fragment: 120-224
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PTTG2
Fragment: 193-202
Site: PBM
Sequence: LPAVCYDIDI
Construct: biotin-ado-ado-LPAVCYDIDI

Average holdup BI: 0.24

Normalized holdup BI: 0.24

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 4.27

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.