Interaction Details


Details of experiments between Q5T2T1 and Q9C0A0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MPP7
Fragment: 120-224
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CNTNAP4
Fragment: 1299-1308
Site: PBM
Sequence: VNENQKEYFF
Construct: Biotin-Ahx-VNENQKEYFF
Average holdup BI: 0.62
Normalized holdup BI: 0.62
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.02
PUBMED ID: 36115835