Interaction Details


Details of experiments between Q5SYB0 and Q9NUP9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.19
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.14
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: LIN7C
Fragment: 85-176
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.52
PUBMED ID: 36115835