Interaction Details


Details of experiments between Q5SYB0 and Q86UT5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 106-208
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.42
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.64
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 106-208
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.47
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 220-307
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 220-307
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 464-550
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: NHERF4
Fragment: 464-550
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835