Interaction Details


Details of experiments between Q5SYB0 and O75970
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.11
Normalized holdup BI: -0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: FRMPD1
Fragment: 1569-1578
Site: PBM
Sequence: TQKFRASTAL
Construct: Biotin-Ahx-TQKFRASTAL

Protein B

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835