Interaction Details


Details of experiments between Q5JV73 and Q9HB71
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FRMPD3
Fragment: 45-133
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CACYBP
Fragment: 219-228
Site: PBM
Sequence: EKQAKGDTEF
Construct: Biotin-Ahx-EKQAKGDTEF
Average holdup BI: 0.1
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.81
PUBMED ID: 36115835