Interaction Details


Details of experiments between Q5JSZ5 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PRRC2B
Fragment: 1-2229
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.85
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
P value: 7.34
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.76
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: PRRC2B
Fragment: 330-344
Site: PxxP_3
Sequence: RLGLSRPLRPLRQLV
Construct: Bio Ado Ado-RLGLSRPLRPLRQLV-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.7
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.18
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: PRRC2B
Fragment: 445-459
Site: PxxP_4
Sequence: VRKAPDPQPPPRKLH
Construct: Bio Ado Ado-VRKAPDPQPPPRKLH-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.45
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: PRRC2B
Fragment: 306-320
Site: PxxP_2
Sequence: PQLRLEPRVPFRQFQ
Construct: Bio Ado Ado-PQLRLEPRVPFRQFQ-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.18
Holdup BI standard deviation: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 2
Measured pKd: 4.11
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: PRRC2B
Fragment: 647-661
Site: PxxP_7
Sequence: QPVYPPPSHPQRTFY
Construct: Bio Ado Ado-QPVYPPPSHPQRTFY-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.1
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.78
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: PRRC2B
Fragment: 251-265
Site: PxxP_1
Sequence: KDPSLRPAQPVRKGA
Construct: Bio Ado Ado-KDPSLRPAQPVRKGA-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: PRRC2B
Fragment: 548-562
Site: PxxP_5
Sequence: QAEKEVPWSPSAEKA
Construct: Bio Ado Ado-QAEKEVPWSPSAEKA-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.06
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: PRRC2B
Fragment: 570-584
Site: PxxP_6
Sequence: AVHKGSPEFPAQETP
Construct: Bio Ado Ado-AVHKGSPEFPAQETP-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.04
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: PRRC2B
Fragment: 848-862
Site: PxxP_8
Sequence: QNLRCSPLEPDFVPD
Construct: Bio Ado Ado-QNLRCSPLEPDFVPD-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.02
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680