Interaction Details


Details of experiments between Q5H9R7 and NC_000001.11
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PPP6R3
Fragment: 1-873
Construct: endogenous protein (K562)

Protein B

Protein: 4945_HAP
Fragment: 203681644-203681669
Construct: Biotin-TCAGCCCTCCGTTTTGTCACCTACAC
Average holdup BI: -0.16
Immobilized partner concentration (10-6M): 5
P value: 2.22
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 2

Protein A

Protein: PPP6R3
Fragment: 1-873
Construct: endogenous protein (K562)

Protein B

Protein: 51_HAP
Fragment: 203681832-203681862
Construct: Biotin-GAATTGAGAGGTATCTTACCGCTCCCACTCC
Average holdup BI: -0.08
Immobilized partner concentration (10-6M): 5
P value: 0.92
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 3

Protein A

Protein: PPP6R3
Fragment: 1-873
Construct: endogenous protein (K562)

Protein B

Protein: 4945_WT
Fragment: 203681644-203681669
Modification: rs10751449/rs10736845
Construct: Biotin-TCAGCCCTCCGTATCGTCACCTACAC
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 5
P value: 0.58
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))

Experiment series 4

Protein A

Protein: PPP6R3
Fragment: 1-873
Construct: endogenous protein (K562)

Protein B

Protein: 51_WT
Fragment: 203681832-203681862
Modification: rs10751451
Construct: Biotin-GAATTGAGAGGTATCTTATCGCTCCCACTCC
Average holdup BI: -0.16
Immobilized partner concentration (10-6M): 5
P value: 1.69
Experiment method: nHU_K562
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))